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Comparative Study of Estimation Methods for Efficient Extraction of Chlorophyll a and Carotenoids Using Different Solvents

Name: Comparative Study of Estimation Methods for Efficient Extraction of Chlorophyll a and Carotenoids Using Different Solvents
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¹Amrish Saini, ²Jyoti Singh, ³Rama Kant*

Bulletin of Pure and Applied Sciences.

Botany, Vol.41 B, No.2.
July-December 2022: P.79-86

Original Research Article

DOI: 10.5958/2320-3196.2022.00009.X

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Categories: 41B(2), JUL-DEC 2022, BPAS-Botany

Description

Comparative Study of Estimation Methods for Efficient Extraction of Chlorophyll a and Carotenoids Using Different Solvents

¹Amrish Saini, ²Jyoti Singh, ³Rama Kant*

Author’s Affiliation

^1,2,3Department of Botany, Chaudhary Charan Singh University Meerut, Meerut, Uttar Pradesh 250001, India.

*Corresponding Author:

Rama Kant

Department of Botany, Chaudhary Charan Singh University, Meerut, Uttar Pradesh 250001, India.

E-mail:

ramakant.algae@gmail.com

Received on 30.01.2022

Revised on 11.05.2022

Accepted on 23.05.2022

Published on 15.12.2022

How to cite this article: Saini A., Singh J. and Kant R. (2022). Comparative Study of Estimation Methods for Efficient Extraction of Chlorophyll a and Carotenoids Using Different Solvents.Bulletin of Pure and Applied Sciences-Botany, 41B(2), 79-86.

Abstract

Abstract Photosynthetic pigments chlorophyll and carotenoids are primary pigments in green plants and algae. These pigments have the potential to turn light energy into chemical energy. In this study, different solvents acetone, ethanol and methanol were used to estimate chlorophyll and carotenoids through UV-VIS spectroscopy from different algal species viz. Anabaena sp., Nostoc sp., Merismopidia sp. And Microcystis sp., Methanol solvent contain the eminent potential for extraction of plant pigments. UV- VIS spectrum (300-800nm) gives the best detection spectrum to evaluate the efficiency of pigments with different solvents. Three methods were used to estimate the best efficiency for the extraction of chlorophyll and carotenoid pigments from algae cells. Results were recorded that Methanol > Ethanol >Acetone in the manner of extraction of plant pigments. Keywords: Algae, Spectroscopy, Solvents, Chlorophyll and carotenoids content.